Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Blume, A.; Weidemann, W.; Stelzl, U.; Wanker, E.E.; Lucka, L.; Donner, P.; Reutter, W.; Horstkorte, R.; Hinderlich, S.
    Domain-specific characteristics of the bifunctional key enzyme of sialic acid biosynthesis, UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (2004), Biochem. J., 384, 599-607.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.60 expressed as His-tag fusion protein in Sf9, and Sf-900 cells Rattus norvegicus
5.1.3.14 expression in Sf9 insect cells, wild-type enzyme and mutant enzymes DELTA1-39, DELTA1-234, DELTA1-356, DELTA383-722, DELTA490-722, DELTA597-722, DELTA697-722, DELTA717-722 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.60 DELTA1-234 monomer molecular mass of 58000 Da, complete loss of epimerase activity, 90% loss of kinase activity Rattus norvegicus
2.7.1.60 DELTA1-359 monomer molecular mass of 45000 Da, complete loss of epimerase activity, almost complete loss of kinase activity Rattus norvegicus
2.7.1.60 DELTA1-39 monomer molecular mass of 79000 Da, complete loss of epimerase activity, 75% loss of kinase activity Rattus norvegicus
2.7.1.60 DELTA383-722 monomer molecular mass of 49000 Da, almost complete loss of epimerase activity, complete loss of kinase activity Rattus norvegicus
2.7.1.60 DELTA490-722 monomer molecular mass of 61000 Da, more than 80% loss of epimerase activity, complete loss of kinase activity Rattus norvegicus
2.7.1.60 DELTA597-722 monomer molecular mass of 72000 Da, almost complete loss of epimerase activity, and kinase activity Rattus norvegicus
2.7.1.60 DELTA697-722 monomer molecular mass of 83000 Da, 25% loss of epimerase activity, 60% loss of kinase activity Rattus norvegicus
2.7.1.60 DELTA717-722 monomer molecular mass of 85000 Da, less than 10% loss of epimerase activity, 20% loss of kinase activity Rattus norvegicus
5.1.3.14 DELTA1-234 mutant enzyme shows no N-epimerase activity Rattus norvegicus
5.1.3.14 DELTA1-356 mutant enzyme shows no N-epimerase activity Rattus norvegicus
5.1.3.14 DELTA1-39 mutant enzyme shows no N-epimerase activity Rattus norvegicus
5.1.3.14 DELTA383-722 epimerase activity is 2% of wild-type enzyme Rattus norvegicus
5.1.3.14 DELTA490-722 epimerase activity is 15% of wild-type enzyme Rattus norvegicus
5.1.3.14 DELTA597-722 epimerase activity is 2% of wild-type enzyme Rattus norvegicus
5.1.3.14 DELTA697-722 epimerase activity is about 70% of wild-type enzyme Rattus norvegicus
5.1.3.14 DELTA717-722 epimerase activity is about 95% of wild-type enzyme Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.60 79000
-
 6 * 79000, His-tagged protein has a mass of 85000 Da, SDS-PAGE, native mass by gel filtration Rattus norvegicus
2.7.1.60 500000
-
 gel filtration Rattus norvegicus
5.1.3.14 85000
-
 6 * 85000, SDS-PAGE Rattus norvegicus
5.1.3.14 500000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.60 ATP + N-acyl-D-mannosamine Rattus norvegicus key enzyme in the synthesis of N-acetylneuraminic acid and therefore of nearly all other sialic acids ADP + N-acyl-D-mannosamine 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.60 Rattus norvegicus
-
-
-
5.1.3.14 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.60 ATP + N-acyl-D-mannosamine
-
 Rattus norvegicus ADP + N-acyl-D-mannosamine 6-phosphate
-
 ?
2.7.1.60 ATP + N-acyl-D-mannosamine key enzyme in the synthesis of N-acetylneuraminic acid and therefore of nearly all other sialic acids Rattus norvegicus ADP + N-acyl-D-mannosamine 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.60 hexamer 6 * 79000, His-tagged protein has a mass of 85000 Da, SDS-PAGE, native mass by gel filtration Rattus norvegicus
2.7.1.60 trimer some of the deletion mutants tends to form trimers Rattus norvegicus
5.1.3.14 hexamer 6 * 85000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.60 UDP-GlcNAc 2-epimerase/ManNAc kinase
-
 Rattus norvegicus
2.7.1.60 UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase two different enzyme activities combined in a single bifunctional enzyme, N-terminal region is for epimerase activity, C-terminal region is for kinase activity Rattus norvegicus
5.1.3.14 UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase bifunctional enzyme Rattus norvegicus